Holographic coherent anti-Stokes Raman scattering bio-imaging
نویسندگان
چکیده
منابع مشابه
Holographic coherent anti-Stokes Raman scattering bio-imaging
CARS holography captures both the amplitude and the phase of a complex anti-Stokes field, and can perform three-dimensional imaging by digitally focusing onto different depths inside a specimen. The application of CARS holography for bio-imaging is demonstrated. It is shown that holographic CARS imaging of sub-cellular components in live HeLa cells can be achieved.
متن کاملInline holographic coherent anti-Stokes Raman microscopy.
We demonstrate a simple approach for inline holographic coherent anti-Stokes Raman scattering (CARS) microscopy, in which a layer of uniform nonlinear medium is placed in front of a specimen to be imaged. The reference wave created by four-wave mixing in the nonlinear medium can interfere with the CARS signal generated in the specimen to result in an inline hologram. We experimentally and theor...
متن کاملHeterodyne coherent anti-Stokes Raman scattering (CARS) imaging.
We have achieved rapid nonlinear vibrational imaging free of nonresonant background with heterodyne coherent anti-Stokes Raman scattering (CARS) interferometric microscopy. This technique completely separates the real and imaginary responses of nonlinear susceptibility chi(3) and yields a signal that is linear in the concentration of vibrational modes. We show that heterodyne CARS microscopy pe...
متن کاملTissue imaging with coherent anti-Stokes Raman scattering microscopy
Sir Chandrasekhara Venkata Raman was justifiably excited when he noticed that when a monochromatic light beam is passed through a simple transparent liquid, different colors can be detected in the scattered light. Raman called this frequency-shifted scattered light ‘a new type of secondary radiation’, because he realized that the phenomenon he discovered was caused by a molecular property diffe...
متن کاملThree-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering
Confocal [1] and multiphoton [2] fluorescence microscopy have become powerful techniques for threedimensional imaging of chemical and biological samples, especially for live cells. This coincides with developments of various natural and artificial fluorescent probes for cellular constituents [3]. For chemical species or cellular components that either do not fluoresce or cannot tolerate labelin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2012
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.3.001744